Splet24. jun. 2011 · Introduction. The stability and turnover of proteins in live cells is a carefully controlled parameter that affects numerous biological functions, which cell cycle, transcriptional control, or DNA damage response are just a few examples of (Hershko and Ciechanover, 1998, Lakin and Jackson, 1999).Additionally, half-life of specific proteins is … SpletTo examine the correlation between the half-life of the EGFP fusion protein and its fluorescence, the EGFP and EGFP-MODC-(422–461) transfected cells used for flow …
A short half-life GFP mouse model for analysis of suprachiasmatic …
SpletWe describe two modifications of the popular reporter green fluorescent protein (GFP) which have short half-lives in our system, the cellular slime mould Dictyostelium discoideum. ... The protein half-life is about 3-5 h. The second construct has an N-terminus derived from the L11 ribosomal protein; it is transported to the nucleus and broken ... SpletThis shorter half-life makes destabilized pFP-DR variants ideal for: Studies that require rapid reporter turnover; Quantitative reporter assays and kinetic studies; Accurately measuring the kinetics of transient mRNA transcription from regulated promoters; Monitoring gene … fat boyz tisch mills menu
Green fluorescent protein: green cells do not always indicate gene ...
Splet27. feb. 2003 · GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted … Splet01. dec. 2016 · Circulating tumor DNA (ctDNA) has a very short half-life in peripheral blood, approximately 1.5 to 2 hours only. ... this experiment doesn't show any GFP expression while transfecting with only ... SpletPlasmid Lentiviral-TOP-dGFP-reporter from Dr. Tannishtha Reya's lab contains the insert LEF-1/TCF-responsive promoter and is published in Nature. 2003 May 22. 423(6938):409-14. This plasmid is available through Addgene. fat boyz towing