Semi-thermal asymmetric reverse pcr starp
WebSTARP marker development. For distinguishing an insertion–deletion of one G between HS2 and 4332, we developed semi-thermal asymmetric reverse PCR markers (STARP), as described by Long et al. with minor modifications. STARP marker included two asymmetrically modified AMAS primers and their same reverse primer. WebForty-nine markers from published sources, four new SSR markers, and five new STARP (semi-thermal asymmetric reverse PCR) markers, were evaluated in BC 2 F 2 populations for assignment of markers to bins. Sr47 was mapped to bin 3 along with 13 markers.
Semi-thermal asymmetric reverse pcr starp
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WebSo far, over 20 Ug99-effective genes have been identified in wheat and its relatives. We recently developed a robust and user-friendly PCR-based method named semi-thermal asymmetric reverse PCR (STARP) for genotyping individual SNPs and indels and initiated a project to develop STARP markers for Ug99-effective genes with high breeding value. WebAug 1, 2024 · Key message: We developed and validated 56 gene-specific semi-thermal asymmetric reverse PCR (STARP) markers for 46 genes of important wheat quality, biotic and abiotic stress resistance, grain...
WebMay 25, 2024 · We developed and validated 56 gene-specific semi-thermal asymmetric reverse PCR (STARP) markers for 46 genes of important wheat quality, biotic and abiotic … WebWe developed and validated 56 gene-specific semi-thermal asymmetric reverse PCR (STARP) markers for 46 genes of important wheat quality, biotic and abiotic stress …
WebMay 28, 2024 · Two, three, and one thermal asymmetric reverse PCR (STARP) markers were developed for Sr7a, Sr8155-B1, and Sr13, respectively. Knowledge of the Sr genes in durum germplasm and the new STARP markers will be useful to pyramid and deploy multiple Sr genes in future durum and wheat cultivars. Introduction WebThree IWGSC scaffold-based simple sequence repeat (SSR) and two SNP-based semi-thermal asymmetric reverse PCR (STARP) markers were developed for the Sr883-2B region, and one STARP marker was developed for Sr883-6A. Sr883-2B was epistatic to Sr883-6A for reaction to TTKSK and TRTTF, and the two genes had additive effects for TMLKC.
WebBased on the polymorphism of FT-D1 between HS2 and 4332, a semi-thermal asymmetric reverse PCR (STARP) marker (SFT-D1) was developed to genotype the RILs (Figure 4b). …
WebAsymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. The technique has applications in some types of … speed dahl coronation streetWebMay 2, 2024 · 5.5. The semi-thermal asymmetric reverse PCR (STARP) markers and their SNP source, sequence, product size and inheritance ..... 103 5.6. Validation of the newly developed markers using 50 durum and common wheat speed d drainWebSelect search scope, currently: catalog all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources speed dance scriptWebSemi-thermal asymmetric reverse PCR (STARP) is one of the SNP genotyping methods developed to reduce operational cost with improved platform compatibility. STARP is a … speed d printspeed daddy long tube headersWebOct 26, 2016 · We show that 5'-complementarity between primers may be exploited in a new PCR method called COMplementary-Primer-Asymmetric (COMPAS)-PCR, using … speed daddy exhaust reviewWebIn contrast, in other fluorescence resonance energy transfer (FRET)-based methods, such as Amplifluor, Kompetitive allele specific PCR (KASP), and semi-thermal asymmetric reverse PCR (STARP), these components are kept separate. speed daddy longtube headers 06 mustang gt