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Plent-ef1a-fh-cmv

WebbpCDH-CMV-MCS-EF1-copGFP载体价格1800元,pCDH-CMV-MCS-EF1-copGFP载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Ampicillin,质粒大小 … Webb15 okt. 2024 · These cells were transfected with the lentiviral expression vector pLent-EF1a-FH-CMV-Puro (EV), pLent-isoform a-flag-his, pLent-isoform b-ha-his, or pLent-isoform c-myc-his. C, Cell Counting Kit-8 (CCK-8) assay investigating the proliferative potential of HT29 cells overexpressing the PD-L1 isoforms. D, Migration ...

Analysis of differentially expressed genes (DEGs) and miRNAs in …

Webbplent‑eF1a‑FH‑cMV‑GP were used to transfected cultured cells (5x105) using Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in DMEM with 10% FBS at a multi‑ plicity of infection of 10 for 20 min. The lentiviral vector with green fluorescent protein (GFP) and resistance tag WebbPlasmid pCDH-EF1-copGFP from Dr. Kazuhiro Oka's lab contains the insert copGFP and is published in Unpublished This plasmid is available through Addgene. ntsb office https://essenceisa.com

Tumor-stroma TGF-β1-THBS2 feedback circuit drives pancreatic …

Webb5 apr. 2016 · 不同病毒载体之间的区别CMV启动插入基因,C端融合Myc-Flag标签)pLent-EF1a-FH-CMV-GFP-P2A-Puro(EF1a启动插入基因,C合Myc-Flag标签;CMV启动荧光报 … Webb1 apr. 2024 · 1. Introduction. Atherosclerosis is a universal problem in modern society that remains the leading cause of mortality worldwide 1, 2, 3.The pathogenesis of atherosclerosis is caused by a combination of risk factors, of which dyslipidemia is a major risk factor for atherosclerotic cardiovascular disease 4, 5.Excessively expanded … WebbDownload scientific diagram Generation of MDBK-GFP and MDBK-GFP-E5 stable cell lines. (A and B) Flow cytometric analysis of GFP-positive MDBK cells stably expressing GFP … nike x tom sachs general purpose shoe

autophagy via MIF/AKT/mTOR axis and hippo-YAP axis malignant …

Category:Rab6c is a new target of miR‑218 that can promote the …

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Plent-ef1a-fh-cmv

pCDH-CMV-MCS-EF1a-GFP+BSD载体图谱质粒图谱、序列、价格、 …

WebbLenti-EF1a (luc-P2A-Puro)-CMV (MCS) (CAT#: Lenti-GTXH007WQ) Datasheet. The product is a lentivirus vector expressing luciferase. Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. This product can be used for the bio-imaging and gene therapay. Specifications. WebbLocate commercially available restriction enzymes by category, name, recognition sequence, or overhang.

Plent-ef1a-fh-cmv

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Webb9 sep. 2024 · 108 MT2A overexpresses lentiviral vector pLent-EF1a-MT2A-FH-CMV-GFP-P2A-Puro 109 (LV-MT2A) and control vector pLent-EF1a-FH-CMV-GFP-P2A-Puro (LV … Webb20 mars 2024 · 维真(Vigene)生物科技有限公司二、操作步骤(一)慢病毒安全操作规范Vigene慢病毒载体是第三代慢病毒载体,其3´LTR的增强子功能发生缺失,形成了自灭活(sefl-inactivating,SIN)LTR,5’LTR换成CMV,是最安全的慢病毒载体。

WebbpLenti-EF1a-EGFP-P2A-Puro-CMV-MCS-3Flag慢病毒表达载体. 质粒干粉 (常温运输,存于-20度,90天保质期,请务必先转化提质粒后使用) ①收到质粒干粉后请先5000rpm离 … Webb精选优质文档-----倾情为你奉上 维真慢病毒系统产品手册 声明 1、Vigene生物科技公司的产品仅限用于研究,不可用于包括人类或体外诊断和治疗在内的其他用途。 2、Vigene生物科技公司的产品,不得修改和转售、转赠给任何第三方,未经Vigene生物科学公司的书面批准,不得将产品用于向其他第三方 ...

Webb21 feb. 2024 · pLenti-EF1a-EGFP-P2A-Puro-CMV-MCS-3Flag 慢病毒质粒 真核表达质粒 原核表达质粒 报告基因质粒 自噬质粒 双分子荧光 抗体表达质粒 基因辑质粒, 酵母相关质粒 …

Webb14 dec. 2024 · Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in …

Webb27 dec. 2024 · Briefly, neuronal cells were infected with MAD2B-overexpressing lentivirus (LV-MAD2B-OE-eGFP) or control lentivirus (LV-eGFP; vector, pLent-EF1a-FH-CMV-GFP-P2A; titer, 1 × 10 8 U/mL) and cultured for another 3 days; neurons with or without lentiviral transduction were treated with CORT (100 μm) for 24 hours for subsequent experiments. nikey cream and black seude shoesWebb22 nov. 2024 · PDF Delta bovine papillomaviruses (δBPVs) causes fibropapillomas or bladder cancer in cattle. E5 is the major oncogene of δBPVs; however, the... Find, read and cite all the research you need ... nike x tiffany \u0026 co air force 1 rafflehttp://www.bioon.com.cn/reagent/Show_product.asp?id=4166982 nike ya youths fleece neck warmer 2.0 blackWebb21 apr. 2024 · The pIRES2-EGFP and pLent-EF1a-FH-CMV-GFP expression vector, packaging plasmid psPAX2 and envelope plasmid pMD2.G were all purchased from … ntsb office of general counselWebb20 juli 2024 · The pLent-4in1-shRNA-GFP-Puro plasmid (Vigene Biosciences, Rockville, MD, USA) was used as a negative control. The stable pdgfd -overexpressing ESCs were … nike x undercover chaos balanceWebbCatalog number: P100021. Description: Lentiviral vector with ampicillin selection and CMV promoter and Myc and FLAG tags. Vector. pLenti-GFP-CMV. Promoter. CMV. nike x tiffany and coWebb8 juli 2024 · pLent-Ef1a-Klf4-P2A-Myc-cmv-C-Puro. 1.87E+09 IU/ml . 同样地,我们对两病毒共感染组与GFP慢病毒对照组中细胞中的四种转录因子mRNA水平的表达情况进行了定 … nike year of the rabbit