Lysotracker染色后固定
Web9 mar. 2015 · LysoTracker® Probes Weakly basic amines selectively accumulate in cellular compartments with low internal pH and can be used to investigate the biosynthesis and … Web20 iun. 2014 · LysoTracker Red is less phototoxic but its rapid photobleaching limits the range of useful applications considerably. We demonstrate that quinacrine is most suitable for long-term imaging when a high number of frames is required. This capacity made it possible to trace acidic vesicles for several hours, during a process of drug-induced …
Lysotracker染色后固定
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http://www.cfdna.com.cn/archives/4469.html WebLysoTracker Deep Red is a cell-permeable, non-fixable, red fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Deep Red has an excitation and …
Web可以的。 注意几点: 1.lysotracker和Hochest都可以染活细胞，直接加进培养基里就行，染完用PBS洗掉，即可拍照。 2.一般lysotracker是红色或者绿色通道的荧光，Hochest是蓝色的，可以兼容。 3.不同细胞着色程度有差异，建议调整下染料浓度和染色时间，第一次染时可以在荧光显微镜下每隔10分钟观察一下，或者请教有经验的师兄师姐。 编辑于 2024-12 … WebLyso-Tracker Red是一种溶酶体(lysosome)红色荧光探针，能通透细胞膜,可以用于活细胞溶酶体特异性荧光染色。 Lyso-Tracker Red为采用Molecular Probes公司的DND-99进行了 …
WebLyso-Tracker Red是一种溶酶体(lysosome)红色荧光探针，能通透细胞膜,可以用于活细胞溶酶体特异性荧光染色。 Lyso-Tracker Red为采用Molecular Probes公司的DND-99进行了荧光标记的带有弱碱性的荧光探针，其中仅弱碱可部分提供质子，以维持pH在中性,可以选择性地滞留在偏酸性的溶酶体中，从而实现对于溶酶体的 ... WebFor confocal, I use lysotracker setting, and laser is 2%, with pin hole slightly larger than 1 airy unit (~1.3um). Digital gain isn't high, and no subtraction since there is no noise other than ...
Web2 sept. 2014 · LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. LysoTracker has been used to detect autophagy-associated lysosomal activity in Drosophila tissues including the fat body, midgut, salivary gland and ovary, as well as in Drosophila cell culture. A low level of LysoTracker …
Web25 sept. 2007 · In order to investigate the relationship of Lysotracker Red to the emergent green fluorescence, we compared the mean fluorescent signal obtained by excitation with a 488nm laser or a 594 nm laser ... supervisory cscs gold cardWebLysoTracker™ Red DND-99 是一种红色荧光染料，可用于标记和追踪活细胞中的酸性细胞器。. LysoTracker™ 探针共有的特点包括：. • 对酸性细胞器具有高度选择性. • 醛固定 … supervisory initiating deviceWebLysoTracker®プローブは、その他の色素またはタンパク質とマルチプレックス化することが可能です。 LysoTracker® Deep Redは、特にRFPおよびGFPのいずれとも適切にマルチプレックス化することができます。 膜タンパク質とLysoTracker®色素は、いずれもリソソームの挙動を追跡するのに効果的なツールです。 選択ガイドを利用してマルチプレキ … supervisory committee audit guideWeb19 aug. 2015 · LysoTracker Green DND-26 is a cell-permeable, non-fixable, green fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Green DND-26 has an excitation and emission maximum of 504/511 nm and can be efficiently excited using a FITC filter. LysoTracker probes are available in a variety of fluorescent … supervisory federal air marshalWebThe flow cytometric use of LysoTracker dyes was employed to investigate the autophagic process and to compare this with the upregulation of autophagy marker, the microtubule-associated protein LC3B. Although the mechanism of action of LysoTracker dyes is not fully understood, they have been used in microscopy to image acidic spherical ... supervisory development course track 3WebLysoTracker™ Deep Red 是一种深红色荧光染料，可用于标记和追踪活细胞中的酸性细胞器。 LysoTracker™ 探针共有的特性包括： • 对酸性细胞器具有高度选择性 • 可有效标记 … supervisory intelligence analyst brian autenWebLysoTracker® Green DND-26 是一种可渗透细胞的绿色染料，能对活细胞中的酸性区室（溶酶体）进行染色。 限制使用 除非 CST 的合法授书代表以书面形式书行明确同意，否书 … supervisory liability 1983 after iqbal