Samples were treated with DNase I (Thermo Scientific) at 37 °C for 1 h to remove any potential residual genomic DNA contamination (one unit of DNase I per 1 μg of RNA). A measure of 1 μg of total RNA was reverse-transcribed using the Superscript First Strand cDNA synthesis kit (Invitrogen) with strand-specific … See more Rosa26-CreERT2 Trf2F/F and Trf2F/+ (ref. 20) MEFs, a gift from Eros Lazzerini Denchi (The Scripps Research Institute, La Jolla, USA), were grown in DMEM supplemented with … See more Total RNA from cultured cells was extracted with RNeasy Mini Kit (Qiagen) for messenger RNA detection, or with mirVana miRNA Isolation kit (Life Technologies) for … See more Rosa26-CreERT mice (Jackson Laboratories) and Trf2 conditional knockout mice33 and mice carrying a p53 conditional allele (Jackson Laboratories) were crossed to generate Trf2/p53/Rosa26 … See more A measure of 1 μg of total cell RNA was reverse transcribed using SuperScript VILO cDNA Synthesis Kit. A volume corresponding to 10 ng of initial RNA was used for each qPCR reaction using GoTaq qPCR Master Mix … See more WebAbstract. DNA double strand breaks (DSBs) are repaired by an extensive network of proteins that recognize damaged DNA and catalyze its repair. By virtue of their similarity, …
A sharp Pif1-dependent threshold separates DNA double-strand
WebSep 11, 2013 · lys2 reporter is inserted in the donor chromosome 16 or 36 kb telomere-proximal from MAT ... we used a disomic yeast system ... BrdU was added 3.5 h after DSB induction. WebTelomeres are the very ends of the chromosomes. They can be seen as natural double-strand breaks (DSB), specialized structures which prevent DSB repair and activation of … free dating personal ads
Higher-order chromatin structure in DSB induction ... - ScienceDirect
WebDNA double strand breaks (DSBs) are repaired by an extensive network of proteins that recognize damaged DNA and catalyze its repair. By virtue of their similarity, the normal ends of linear chromosomes and internal DNA DSBs … WebJul 24, 2015 · Thus, kinesin-14 displays increased localization to the subtelomeric DSB upon its induction in a manner that is likely dependent on cohibin-dependent … WebNov 1, 2016 · Induction of DSBs at telomeres has been shown to stimulate interchromosomal recombination and telomere clustering in ALT cells, but not in … blood red carpet