WebJul 1, 2002 · Spectrophotometric methods for determining the activity of cytokinin oxidase/cytokinin dehydrogenase (EC 1.5.99.12) were developed and optimized. A …
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WebApr 10, 2024 · Sugarcane is a complex polyploid aneuploid cash crop, and transgenic varieties are important for molecular genetic and traditional breeding approaches. Herein, the sugarcane variety ROC22 served as the receptor, the Bar gene served as a screening marker, and positive and negative fragments of the ScD27.2 gene, upstream of … WebFeb 4, 2024 · We generated transgenic poplar harboring cytokinin oxidase/dehydrogenase ( CKX)2, a gene encoding a cytokinin degrading enzyme, driven by the phloem-specific CLE41b promoter, indicating that …
WebAug 21, 2007 · Transgenic tobacco plants overexpressing single Arabidopsis thaliana cytokinin dehydrogenase (CKX, EC 1.5.99.12) genes AtCKX1, AtCKX2, AtCKX3, AtCKX4, AtCKX5, AtCKX6, and AtCKX7 under the control of a constitutive 35S promoter were tested for CKX-enzymatic activity with varying pH, electron acceptors, and substrates. This … WebJul 11, 2024 · Cytokinins constitutes a vital group of plant hormones regulating several developmental processes, including growth and cell division, and have a strong influence on grain yield. Chemically, they are the derivatives of adenine and are the most complex and diverse group of hormones affecting plant physiology.
WebDec 7, 2015 · Here, we show that the Lotus japonicus Ckx3 cytokinin oxidase/dehydrogenase gene is induced by Nod factor during the early phase of nodule initiation. ... Quantification was obtained using a multiple reaction-monitoring mode of selected precursor ions and the appropriate product ion. GenBank accession numbers: … WebAn enzyme degrading cytokinins with isoprenoid side chain, previously named cytokinin oxidase, was purified to near homogeneity from wheat and barley grains. New techniques were developed for the enzyme activity assay and staining on native electrophoretic gels to identify the protein.
WebMar 29, 2024 · Cytokinins are one of the five major hormones families in plants and are important for their normal growth and environmental adaptability. In plants, cytokinins are mostly present as glycosides in …
WebJul 25, 2024 · The aim of this study was to investigate the action spectrum of two urea derivatives, the 1,3-di(benzo[d]oxazol-5-yl)urea (5-BDPU) and the 1,3-di(benzo[d]oxazol-6-yl)urea (6-BDPU). In order to evaluate a possible adjuvant activity on cytokinins the compounds alone or in the simultaneous presence of different cytokinins were assayed … graveyard keeper how to move workstationsWebbehaves as a dehydrogenase rather than an oxidase. This was confirmed by the ability of the enzyme to transfer electrons to artificial electron acceptors, such as phen-azine methosulfate and 2,6-dichlorophenol-indophenol. 2,3-Dimethoxy-5-methyl-1,4-benzoquinone, a precursor of the naturally occurring electron acceptor ubiquinone, graveyard keeper how to make black paintTo identify an omnipresent FAD-dependent protein, that could be related to the cytokinin binding domain we performed a pBlast search. By application of pBlast search, we have found that on the sequence level the closest to CKX protein in Arabidopsis thalianais the FAD-linked D-2-hydroxyglutarate … See more To track the evolutionary origin of the CKXs, we have searched for the homologous sequences from the Uniprot, Pfam, and NCBI databases using the homology-based … See more Because our phylogenetic tree analysis could not answer the question regarding the origin of the cytokinin binding domain, we have analysed structural models of the CK-binding domains. … See more Domain architecture plays a key role in understanding the functionality of proteins along with their evolutionary history. To evaluate the gain … See more To understand the evolutionary history of the C-terminal cytokinin binding domain proteins the phylogenetic trees were inferred with the … See more choc n roll coventryWebJun 1, 2024 · Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the … chocnroll reims facebookWebMay 1, 2005 · Cytokinin dehydrogenase was confirmed to be present in the apoplast of cells. Co-staining of enzyme activity for laccase, an enzyme poised to function on the cell wall in the apoplast, in those tissues suggests a possible cooperation of the enzymes in cytokinin degradation. graveyard keeper how to make hand mixerWebJan 31, 2024 · Cytokinin is first perceived by hybrid histidine kinase (HK) receptors, mainly localized in endoplasmic reticulum (ER), and results in autophosphorylation ( Anantharaman and Aravind, 2001; Caesar et al., 2011; Lomin et al., 2011; Wulfetange et al., 2011 ). graveyard keeper how to sell cratesWebApr 11, 2024 · In addition to cytokinin, Zn deficiency also represses the levels of auxin and gibberellic acid (GA) in wheat and maize (Sekimoto et al., 1997) and abscisic acid (ABA) levels in rape leaves (Cakmak et al., 1989), whereas it induces the accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC), a precursor of ethylene biosynthesis in … chocnroll