Cytokinin dehydrogenase activity
WebSep 16, 2015 · Cytokinin dehydrogenase activity was measured using a modified end-point method described earlier . The reaction mixture (total volume of 0.6 ml in 1.5 ml tube) consisted of 100 mM McIlvaine buffer, pH 5.0 or 7.5, electron acceptor, substrate (dissolved in dimethyl sulfoxide that reached the final concentration of 2.5% in the reaction mixture ... WebSep 28, 2007 · The biological activity of cytokinin nucleotides is still unclear, since they usually show no activity in bioassays but can bind to certain cytokinin receptors (Spichal et al., 2004). The adenine moiety of cytokinins can also be glycosylated, resulting in the formation of either N 7 - or N 9 -glucosides, which are much less active than the ...
Cytokinin dehydrogenase activity
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WebApr 9, 2024 · Because CKme- tabolism modifying adeninemoiety par-tially shared purinesalvage pathway, nucleobase CKs readilyconverted correspondingnucleotides nucleosides(31). metabolicflow plays importantrole activeCKs (31). con-text, reduc-tion CKXactivity Habataki,NIL-Gn1a, increasedproduction CKconjugates overallCK activity … WebMay 1, 2005 · Fig. 3 Histochemical detection of cytokinin dehydrogenase activity in maize seedlings (2 weeks after germination). (A) Stem cross-section and (B) close-up of phloem cells. Left and right panels show activity staining of the sections with iP substrate and control experiments without iP, respectively. The activity is expressed as purple ...
WebSep 1, 2007 · Variable distribution of cytokinin oxidase/dehydrogenase activity (CKX: EC 1.5.99.12) in leaves of young pea seedlings (cv. Manuela) during the period needed for full leaf bud expansion was observed. WebEven though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry ...
WebThe present study reviews relevant research pertaining to the biological activity of the CKX enzyme in the context of adapting towards abiotic stresses along with improved grain … WebThe Cytokinin Oxidase/Dehydrogenase CKX1 Is a Membrane-Bound Protein Requiring Homooligomerization in the Endoplasmic Reticulum for Its Cellular Activity Degradation …
WebMar 24, 2010 · Almost 80% of 52 regenerated lines of Golden Promise exhibited significantly decreased cytokinin oxidase/dehydrogenase (CKX) enzyme activity in bulked samples of their T 1 roots. There was a positive correlation between the enzyme activity and the plant productivity, expressed as the yield, the number of seeds per plant, and the 1000 grain …
In enzymology, a cytokinin dehydrogenase (EC 1.5.99.12) is an enzyme that catalyzes the chemical reaction N -dimethylallyladenine + electron acceptor + H2O adenine + 3-methylbut-2-enal + reduced acceptor The 3 substrates of this enzyme are cytokinin (here represented by N -dimethylallyladenine), electr… inarritu net worthWebMay 15, 2012 · Cytokinin dehydrogenase (CKX) catalyzes the irreversible degradation of cytokinins (CKs). CKs play a role in the regulation of hydrogen peroxide (H 2 O 2) accumulation while H 2 O 2 is involved in chlorophyll degradation. Here, we elucidated how CKX differentially regulates representative CK levels and indirectly influence H 2 O 2 … inarrearWebApr 9, 2024 · N 6-(2-Isopentenyl)adenine (2iP) and zeatin are natural N 6-substituted purine cytokinins that are used for in vitro culture protocols, mainly to promote axillary shoot proliferation and adventitious shoot induction.One of the mechanisms that regulates local cytokinin homeostasis in the in vitro plant is their degradation by cytokinin … incheon timeWebNov 8, 2024 · The dominant domain architecture associated with the cytokinin dehydrogenase consists of N-terminal FAD-binding (PF01565) (app 200aa) and C … inaros weaponWebJan 5, 2024 · Cytokinin regulates the activity of reproductive meristems, flower organ size, ovule formation, and thus seed yield in Arabidopsis thaliana. Bartrina I, et al. Plant Cell, … incheon to alpensiaWebThe larvicidal activity of natural inspired piperine-based dienehydrazides against Culex pipiens: ... Transcriptome profiling reveals cytokinin promoted callus regeneration in Brassica juncea: ... A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain: inars 看護WebCytokinin degraded by CKX1 is required for cell division in the female gametophyte by modulating the expression of cell cycle genes (PubMed:35995110). 5 publications … inarritu\u0027s work