Cpg stimulation of human monocytes
WebUpon stimulation with a suboptimal concentration of CpG-ODN 2216 (0.1 µM), 1 µM of LEN enhanced pDC-derived IFN-α production (185% of vehicle control culture; p=0.023) . These results indicated that exposure to a clinical concentration of LEN sustained and potentially enhanced, IFN-α production by pDCs in response to weak CpG stimulation. WebJun 2, 1983 · Cultured human monocytes produce a non-prostanoid factor which stimulates bone resorption in vitro. Production of the factor is not dependent on prostaglandin synthesis, but stimulation of resorption by the factor does involve prostaglandin production by bone. Salmon calcitonin inhibits the stimulatory activity by up …
Cpg stimulation of human monocytes
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WebCurrent results demonstrate that the recently identified "D" type of CpG ODN stimulate human peripheral blood monocytes to mature into functionally active DC over 2-4 days. … WebJul 15, 2007 · In this study, we report that the UT receptor is expressed and functional on human PBMC and rat splenocytes. PBMC surface expression of the UT receptor was mainly found in monocytes and NK cells, also in a minority of B cells, but not in T cells. Stimulation of monocytes with LPS increased UT receptor mRNA and protein expression.
WebApr 8, 2016 · Reminiscent of conditions in mice, also HCMV-stimulated human pDC mount substantial IFN-α responses, while pDC are largely resistant to HCMV infection when compared with either CD11c + DC or freshly isolated monocytes [28,29]. Treatment of human pDC with TLR7/9 inhibitory CpG oligodinucleotides (ODN) abrogated HCMV … WebApr 13, 2024 · The human THP-1 monocyte line and human hepatocellular carcinoma cell line HepG2 cells were obtained from the Cell Centre of Peking Union Medical College. HUVECs were maintained in growth medium including 1/3 endothelial cell medium (ECM) (ScienCell) and 2/3 M199 medium (Gibco) supplemented with 10% (v/v) foetal bovine …
WebJun 27, 2024 · Dendritic cells (DC) and monocytes are vital for the initiation of innate and adaptive immune responses. Recently, we identified bona fide DC subsets in blood of cattle, revealing subset- and species-specific transcription of toll-like receptors (TLR). In the present study, we analyzed phenotypic and transcriptional responses of bovine DC … WebFeb 2, 2012 · Monocytic MDSC express TLR9 and respond to CpG stimulation by i) losing their ability to suppress T cell function, ii) producing Th1 cytokines and iii) differentiating into macrophages with tumoricidal capability. ... which is a lung epithelial tumor cell line that expresses the E7 oncoprotein from human papilloma virus 16; and 4T1 ...
WebMay 14, 1999 · Human PBMC (1 × 10 6 cells/ml) were incubated for 4 h with either LPS (10 ng/ml to 0.006 ng/ml) or DNA samples. Protein secretion was blocked with brefeldin A (1 …
WebJun 4, 2024 · Introduction. Monocytes are innate immune cells that account for ~10% of nucleated blood cells (1, 2).They play a key role in anti-microbial immunity through direct responses including phagocytosis and cytokine production in addition to regulating other cells of the innate and adaptive immune systems ().Under inflammatory conditions blood … flutter axisdirectionWebApr 15, 2003 · In this study, we investigated CpG-dependent IP-10 production. CpG-A ODN were found to be potent inducers of IP-10 secretion, effecting pDC directly, while in monocytes the effect was indirect and mediated by pDC-derived IFN-α. We found no evidence for direct stimulation of highly purified human monocytes by any CpG ODN. flutter aws iot coreWebAbout. Motivated immunologist with core expertise in inflammation, autoimmunity and immune-oncology. PhD from Buenos Aires University … flutter azure ad authenticationWebDec 1, 2006 · Here, we show that human monocyte‐derived dendritic cells (moDC) contain TLR9 protein, surprisingly, in amounts comparable with plasmacytoid DC (pDC). Immature moDC but not mature moDC nor monocytes captured CpG‐ODNs. moDC stimulation with the CpG‐A ODN D19 up‐regulated CD83, CD86, and HLA‐DR. flutter azure ad b2c with googleWebDec 21, 2024 · Interestingly, human monocyte-derived DCs (moDC) triggered via RIG-I show a commitment to glycolysis to promote type I IFN production and T cell priming in contrast to pDCs. Our findings reveal for the first time, that pDCs display a unique metabolic profile; TLR9-driven but not RIG-I-mediated activation of pDCs requires glycolytic … green gravity chairWebPreterm infants are more susceptible to severe bacterial and viral infectious diseases than their full-term counterparts. A major contributor to this increased susceptibility may be … flutter back button colorWebshow that human monocyte-derived dendritic cells (moDC) contain TLR9 protein, surprisingly, in amounts comparable with plasmacytoid DC (pDC). Immature moDC but not mature moDC nor mono-cytes captured CpG-ODNs. moDC stimulation with the CpG-A ODN D19 up-regulated CD83, CD86, and HLA-DR. Without CD40 ligand costimulation, … green gravity australia